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KMID : 0903519850280030162
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1985 Volume.28 No. 3 p.162 ~ p.166
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Substrate Specificity of Cellulase from Aspergillus niger
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신현경/Shin, Hyun Kyung
김재욱/박관화/오태광/Kim, Ze Uook/Park, Kwan Hwa/Oh, Tae Kwang
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Abstract
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Three isozymes of Carboxymethyl Cellulase (FⅠ^*, FⅡ^*, FⅢ) and two fractions of β-1,4-D-Cellobiohydrolase(CⅠ, CⅡ) from Aspergillus niger were purified by Sephadex G-150, DEAE-Sephadex and Sephadex G-75 column chromatography.
From the results of enzymatic hydrolysis and X-ray diffraction, β-1,4-D-Cellobiohydrolase has a high activity toward highly crystalline cellulose such as filter paper and acts synergistically with Cx enzyme.
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